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描述
Single-cell
Sequanta Technologies Single-cell Multi-omics Solutions
Provide end-to-end services for single-cell genomic, transcriptomic and proteomic studies
As single-cell technology becomes more sophisticated, multi-omics data analysis by integrating genomic, transcriptomic, and proteomic studies allows for new scientific insights into biological mechanisms. Sequanta Technologies boasts state-of-the-art single-cell technology platforms enabling multi-omic studies in tumor immune microenvironment, immune profiling, stem cell differentiation, cell therapies and other fields.
Comprehensive Single-cell Technology Platforms
Transcriptome + Proteome
  • 1
    10× Genomics High throughput Transcriptome + Proteome
  • 2
    Singleron High throughput Transcriptome + Proteome
  • 3
    10× Genomics Visium Spatial Transcriptome + Proteome
  • 新增设备
    Takara SMART-Seq Low throughput Transcriptome
Genome + Proteome
  • 5
    Missionbio Tapestri High throughput Genome + Proteome
  • 6
    MDA Low throughput Genome
  • 7
    MALBAC Low throughput Genome
Application scenario
Tumor

• Composition of tumor microenvironment

• Mechanisms of tumor development

• Organ cell atlas

• Surface proteins

Immunity

• TCR/BCR composition and clonal diversity

• Immune cell differentiation and pathway-related research

• Dynamic monitoring of lymphocytes

• Antibody screening

Clinical Applications

• Diagnosis optimization

• Efficient detection of treatment response and prognostic guidance

• Single-cell panel detection

• Relationship between efficacy and variation and improvement of treatment regimes

Drug Research and Gene Therapy

• Immunotherapy

• Cell therapy

• Target screening

• Vaccine research

• Resistance mechanism research

Single-cell Solution I: Single-cell Transcriptome + Proteome
Search for new cell subpopulations in the tumor microenvironment and reveal their functions
Tumor immunotherapy has greatly prolonged the survival cycle of tumor patients, but not all tumor patients respond to immunotherapy in Clinical manifestions. The reason is the high heterogeneity of tumor microenvironment, so it is crucial to analyze the tumor microenvironment to improve the effectiveness of tumor immunotherapy. ScRNA-seq can unsupervisedly isolate similar cell subpopulations from single-cell gene expression data of unknown identity, and infer the composition of the tumor microenvironment and the functions of all cell types based on the results of biological calculations, databases, and feature genes.
描述
Figure Single-cell cluster analysis: cell populations t-SNE clustering and characteristic gene heat of cell populations
Explore immune cell composition and screen for highly effective specific antibodies

The study of immune cell diversity and immune cell microenvironment based on single-cell immunome libraries can be used for drug guidance and prediction of immunotherapy efficacy, and the single-cell immunome library technology, as an important tool for studying immune response, can also be used for vaccine development.

描述
Figure Single-cell immunome library analysis: B-cell subpopulation analysis, efficacy prediction marker analysis
The polymer is designed to link the antigen via biotin to the corresponding barcode sequence. Binding VDJ to the sequence of feature barcode allows to acquire the correspondence between BCR sequence and target antigen, and to achieve the purpose of high precision antibody screening.
描述
Figure Antibody screening by single-cell sequencing
Technology platform:

10× Genomics, Singleron

Diversified services:

3’ RNA, 5’ RNA, TCR/BCR, ATAC, CRISPR Screening

Single-cell Solution II: Spatial Transcriptome + Proteome
Identify cell types and clarify cell spatial location and interactions

Spatial transcriptome sequencing technology can provide both spatial location and gene expression of cells. Based on the combination of cell spatial localization and single-cell sequencing techniques, it can more be precise to annotate cell types and reveal cellular interactions.

描述
Figure Spatial transcriptome data analysis: cell spatial distribution, cell clustering analysis, and characteristic gene analysis of cell populations
Technology platform：

Genomics Visium

Support FFPE samples
Single-cell Solution III: Single-cell Genome + Proteome

With Mission Bio Tapestri, the only commercially available high-throughput single-cell genomic and proteomic technology platform, up to 10,000 cells can be captured and 20-1,000 genomic target regions can be detected in a single experiment, while cell surface protein expression can be analyzed using antibodies to detect SNV, InDel and CNV at the single-cell level.

Hematologic studies

Clonal evolution, resistance mechanisms, residual lesions

Gene editing

CRISPR on-/off-target, accurate assessment of gene editing efficiency, rapid detection of transduction efficiency

Cell and gene therapy

Knock-in/knock-out validation, detection of editing and off-target rates, detection of chromosomal translocations, binding analysis, transduction efficiency evaluation, vector copy number determination

Solid tumor studies

Clonal evolution, resistance mechanisms, chromosome copy number changes

Biomarker studies

Single-cell panel detection, relationship between efficacy and variation, and improvement of treatment regimes

描述
Figure Single-cell genome analysis: tumor clonal diversity and mutational evolutionary patterns

  • Vector

  • Gene Editing

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Figure Precise identification of vector transfection and gene editing results in cell therapy at the single-cell level
Superior sequencing service accelerating new single-cell discoveries

Sequanta Technologies has launched the automated high-throughput sequencing service production node “Flash Sequencing Store” based on digital technology. It now has four labs in Shanghai, Guangzhou and Beijing, allowing effective single-cell library sequencing service for all customers with different needs, providing fast and high-quality one-stop sequencing service

描述
Contact us
240 Hedan Road, Waigaoqiao Pilot Free Trade Zone, Shanghai, China
Shanghai International Biomedical Science & Innovation Center info@sequanta.com
021-20500599
Copyright: Shanghai Sequanta Technologies Co., Ltd. Record No.: Shanghai
ICP Record No. 2021028059-1